Micro Cell Culture Method for Isolation of Chlamydia trachomatis
نویسندگان
چکیده
منابع مشابه
Micro cell culture method for isolation of Chlamydia trachomatis.
Presterilized mictotiter plates (96 wells) with BHK-21 cells on 5-mm cover slips were successfully used for cell culture isolation of trachoma from 15 infected conjunctival scrapings.
متن کاملNeutralization of Chlamydia trachomatis in cell culture.
Neutralization of Chlamydia trachomatis was assayed by the decrease in inclusion-forming units in baby hamster kidney cells grown in culture. Five percent fresh guinea pig sera increased neutralization titers of rabbit antisera 100- to 1,000-fold but had no effect when normal rabbit sera were tested. Neutralization of a type A or B trachoma isolate was strain specific. Neutralization by human e...
متن کاملMicro direct inoculation method for the isolation and identification of Chlamydia trachomatis.
Current standard procedures for the isolation and identification of Chlamydia trachomatis are laborious. A more rapid method for the isolation and identification of C. trachomatis has been developed. This method features the use of a multiwell microplate to which trypsinized McCoy cells and specimens are added simultaneously, followed by centrifugation. A simplified one-step iodine-glycerol sta...
متن کاملScreening for Chlamydia trachomatis among Infertile Women Using PCR Method
Background & objectives: Chlamydia trachomatis is a gram negative bacterium and chlamydia infection, as a curable infection, is one of the most common sexually transmitted diseases (STD). With regard to the essential role of chlamydia in infertility, the study of the prevalence of asymptomatic cases is precious. The aim of this study was to determine of the prevalence of chlamydia trachomatis...
متن کاملDetecting Chlamydia trachomatis in men with urethritis: serology v isolation in cell culture.
The accuracy of single serovar (L2) inclusion immunoperoxidase assay (IPA) to show serum IgG and IgA antibodies specific to chlamydiae was compared with culture for Chlamydia trachomatis to diagnose chlamydial infection in 73 men with acute urethritis. C trachomatis only was isolated from 18 (25%), Neisseria gonorrhoeae only from 17 (23%), and both organisms from six (8%). Thus 24 (33%) yielded...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.28.4.727-729.1974